Novel synthetic chemically-modified functional nucleic acids for therapeutic and diagnostic ​applications

We synthesise and develop novel chemically-modified therapeutic nucleic acids such as antisense oligonucleotides (AOs), DNAzymes, siRNA, antimiRs, molecular beacons and aptamers capable of targeting pathological RNAs and proteins, and investigate their potential as ‘theranostics’ for tackling solid cancers, neuromuscular diseases, metabolic diseases and infectious diseases. Towards this, we develop novel nucleic acid analogues with superior properties and unique conjugation chemistries and synthesise smart oligonucleotide constructs via solid-phase oligonucleotide synthesis using phosphoramidte chemistry. Our laboratory has two state-of-the-art oligonucleotide synthesisers capable of synthesising oligonucleotides from small to large scales with various nucleic acid chemistries.   

Chemical synthesis of oligonucleotides

Therapeutic applications of nucleic acids

Strutural presentations of conventional and chemically-modified nucleic acid monomers

Representative Publications:

1. Le BT, Chen S, Abramov M, Herdewijn P, Veedu RN (2016). Evaluation of Anhydrohexitol nucleic acid, Cyclohexenyl nucleic acid and D-Altritol nucleic acid-modified 2’-O-methyl RNA mixmer antisense oligonucleotides for exon skipping in vitro. Chemical Communication, 52, 13467-13470.
2. Le BT, Filichev VV, Veedu RN (2016). Investigation of twisted intercalating nucleic acid (TINA)-modified antisense oligonucleotides for splice modulation by induced exon-skipping in vitro. RSC Advances, 6 , 95169-95172.

3. Kim KT, Veedu RN, Seo YJ, Kim BH (2014). Design of Quencher-free Molecular Beacons for Probing CAG Repeat Oligonucleotides. Chemical Communications, 50, 1561-1563.
4. Morihiro K, Kodama T, Tefu K, Moai Y, Veedu RN, Obika S (2013). Selenomethylene-Locked Nucleic Acid (SeLNA) Enables Reversible Hybridization in Response to Redox Changes. Angewandte Chemie International Edition, 52, 5074-5078.